Figure 3.

EZH2 knockdown increased cell apoptosis. (A) Induction of apoptosis was examined by Annexin V/PI double staining using a FACS analysis. (B) The marker proteins of apoptosis were detected by western blot analysis and RT-qPCR. The promoting apoptosis protein was increased and the anti-apoptosis protein was decreased. Results shown are from 3 independent experiments. **P<0.01 vs. the control group. EZH2, enhancer of zeste homolog 2; RT-qPCR, quantitative reverse transcription-polymerase chain reaction; PI, propidium iodide; Bax, BCL2 associated X.