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. 2018 Apr 12;15(6):4599–4614. doi: 10.3892/etm.2018.6053

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Copyright: © Feng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Sal pretreatment downregulates apoptosis in ConA-induced liver injury. (A) Relative mRNA levels of Bcl-2, Bax, caspase-3 and caspase-9. (B and C) Protein expression of Bcl-2, Bax, caspase-3 and caspase-9. (D) Immunohistochemistry was used to detect Bcl-2 and Bax (original magnification, ×200). The ratio of brown area to total area was analyzed with Image-Pro Plus software 6.0. (E) TUNEL staining indicated apoptotic cells in the four groups at 8 h after ConA injection (original magnification, ×200). The percentage of TUNEL-positive cells was determined by Image-Pro Plus software 6.0. Data are presented as the mean + standard deviation (n=6). *P<0.05 vs. NC; ^#P<0.05 vs. ConA (25 mg/kg); ^†P<0.05 vs. Sal (10 mg/kg) + ConA (25 mg/kg). Sal, salidroside; ConA, concanavalin A; NC, negative control; Bcl-2, B-cell lymphoma-2; Bax, Bcl-2-associated X protein.