Table I.
Primary antibodies used in the current study.
| Antibody | Type | Species | Targeted species | Dilution ratio (western blot/IHC) | Supplier | Catalog number |
|---|---|---|---|---|---|---|
| β-actin | Mono | M | H, M, R | 1:1,000 | CST | #3700 |
| IL-1β | Mono | R | M | 1:1,000 | CST | #12507 |
| IL-6 | Poly | R | H, M, R | 1:1,000/1:200 | PT | 21865-1-AP |
| TNF-α | Mono | M | H, M, R | 1:1,000/1:200 | PT | 60291-1-Ig |
| Bcl-2 | Mono | M | H, M | 1:500/1:200 | Ab | ab692 |
| Bax | Poly | R | H, M, R | 1:1,000/1:200 | PT | 60267-1-Ig |
| Caspase-3 | Poly | R | H, M, R | 1:1,000 | PT | 19677-1-AP |
| Caspase-9 | Mono | M | H, M, R | 1:1,000 | PT | 66169-1-Ig |
| Beclin-1 | Poly | R | H, M, R | 1:1,000/1:200 | PT | 11306-1-AP |
| LC3 | Poly | R | H, M, R | 1:500/1:200 | PT | 14600-1-AP |
| p62 | Poly | R | H, M, R | 1:1,000 | PT | 55274-1-AP |
| PI3K | Poly | R | H, M, R | 1:1,000/1:200 | PT | 20584-1-AP |
| Akt | Mono | M | H, M, R | 1:2,000 | PT | 60203-2-Ig |
| p-Akt | Mono | M | H, M, R | 1:1,000/1:200 | PT | 66444-1-Ig |
IHC, immunohistochemistry; IL, interleukin; TNF, tumor necrosis factor; Bcl-2, B-cell lymphoma-2; Bax, Bcl-2 associated X protein; LC3, microtubule-associated protein 1A/1B-light chain 3; PI3K, phosphoinositide 3-kinase; p-, phosphorylated; H, human; M, mouse; R, rabbit; CST, Cell Signaling Technology, Inc. (Danvers, MA, USA); PT, ProteinTech Group, Inc. (Chicago, IL, USA); Ab, Abcam (Cambridge, MA, USA).