Table I.

PCR conditions and primers sequence used for the amplification of putative oncogenes involved in thyroid cancer.

Gene	Exons	5′→3′ Sequence	Tm	Lenght	Gene	Exons	5′→3′ Sequence	Tm	Lenght
CTNNB1	EX3	TCACTGAGCTAACCCTGGCTAT	60	497	TERT	EX2	CTACTCCTCAGGCGACAAGG	60	197
		TCTCTTTTCTTCACCACAACATTT					CAAGCAGCTCCAGAAACAGG
PIK3CA	EX10	TGCTTTTTCTGTAAATCATCTGTGA	61	285	AKT	EX1	TAGAGTGTGCGTGGCTCTCA	60	171
		TGCTGAGATCAGCCAAATTC					CGCCACAGAGAGAAGTTGTTGA
	EX20	TGACATTTGAGCAAAGACCTG	60	387	H-RAS	EX2	GTGGGTTTGCCCTTCAGAT	60	325
		TGTGGAATCCAGAGTGAGCTT					CCTATCCTGGCTGTGTCCTG
TP53	EX5/6	CACTTGTGCCCTGACTTTCA	Touch	464	H-RAS	EX3	AGAGGCTGGCTGTGTGAACT	60	344
		CTTAACCCCTCCTCCCAGAG	70/55				ACATGCGCAGAGAGGACAG
	EX7	CTTGGGCCTGTGTTATCTCC	Touch	228	N-RAS	EX2	GAAAGCTTTAAAGTACTGTAGATGTGG	60	240
		TGGAAGAAATCGGTAAGAGGTG	70/55				CCGACAAGTGAGAGACAGGA
	EX8/9	CAAGGGTGGTTGGGAGTAGA	Touch	545	N-RAS	EX3	TTGCATTCCCTGTGGTTTTT	60	325
		ACCAGGAGCCATTGTCTTTG	70/55				TGGTAACCTCATTTCCCCATA
PTEN	ex5	TGCAACATTTCTAAAGTTACCTACTTG	60	397	K-RAS	EX2	TTAACCTTATGTGTGACATGTTCTAA	60	230
		AAACCCAAAATCTGTTTTCCAA					ATCAAAGAATGGTCCTGCAC
	ex6	GGCTACGACCCAGTTACCAT	60	347	K-RAS	EX3	TCAAGTCCTTTGCCCATTTT	60	375
		CCTGCATAAATTTCAAATGTGG					TGCATGGCATTAGCAAAGAC
	ex7	TCCATATTTCGTGTATATTGCTGA	60	397	ALK	EX23	TTCCTCCCAGTTTAAGATTTGC	60	298
		GCAAAACACCTGCAGATCTAA					CATCGAGGAACTTGCTACCC
	ex8	TGATAGTTTATTTTGTTGACTTTTTGC	60	452	EML4/	EX23/0	GTGCAGTGTTTAGCATTCTTGGGG	60	244
		GTCAAGCAAGTTCTTCATCAGC			ALK	EX2	TGCCAGCAAAGCAGTAGTTG
BRAF	EX15	TTGACTCTAAGAGGAAAGATGAAGTACT	60	320	STRN/	EX3/	CGGGACAGAATTGAATCAGGG	60	244
		AGCATCTCAGGGCAAAAAT			ALK	EX20	TGCCAGCAAAGCAGTAGTTG
TERT	Promoter	CTGGCGTCCCTGCACCCTGG	62	474	ALK	EX27/	ACCCAAGAACTGCCCTGG	60	233
		ACGAACGTGGCCAGCGGCAG	Q-SOL			EX29	GAGAGACCAGGAGAGGAGGA

PCR, polymerase chain reaction; Tm, melting temperature.