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Methylation-specific amplification primers for the MGMT promoter.
| Primers | Sequence (5′-3′) | Products, bp | Annealing temperature, °C |
|---|---|---|---|
| MGMT-M | F: TTTCGACGTTCGTAGGTTTTCGC | 83 | 59 |
| R: GCACTCTTCCGAAAACGAAACG | |||
| MGMT-U | F: TTTGTGTTTTGATGTTTGTAGGTTTTTGT | 91 | 59 |
| R: AACTCCACACTCTTCCAAAAACAAAACA |
MGMT, O6-methylguanine-DNA methyltransferase; F, forward; R, reverse; M, methylated primer; U, un-methylated primer.
