Figure 3.

Physical interaction between GRP78 and CD24. (A) Upper panel: IP with CD24 using anti-CD24 antibody and IB detection with anti-GRP78 antibody. Lower panel: IP with GRP78 using anti-GRP78 antibody and IB detection with anti-CD24 antibody. Whole-cell lysates of HT29 cells were subjected to IP. The levels of GRP78 and CD24 in the resulting precipitates were determined by western blot analysis. IgG was used as a control albumin. (B) Whole-cell lysates from HT29 cells with or without L-OHP treatment (5 µM, 24 h) were subjected to IP with CD24 antibodies. The levels of GRP78 and CD24 in the resulting precipitates were determined by western blot analysis. Images are representative of three independent experiments. GRP78, glucose-regulated protein 78; IP, immunoprecipitation; IB, immunoblot; IgG, immunoglobulin G.