Figure 2.

Fbxw8 is targeted by miR-3160-5P following binding to the 3′-UTR. (A) RT-qPCR was used to assess the upregulated expression of miR-3160-5P in DU145 cells transfected with miR-3160-5P mimics, compared with controls or miR-SCR-transfected cells. Fbxw8 (B) mRNA and (C) protein expression levels in DU145 cells that were transfected with miR-3160-5P or miR-SCR were assessed by RT-qPCR and western blot analysis. (D) The predicted miR-3160-5P binding site in the Fbxw8 3′-UTR and its mutated version due to site mutagenesis. (E) The relative luciferase activities of the WT and Mut Fbxw8 3′-UTR regions (mean ± SD of three independent experiments, performed in triplicate). **P<0.01. The mean ± SD of three representative experiments are presented. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; miR, microRNA; miR-SCR, scramble microRNA; Fbxw8, F-box and WD repeat domain containing 8; 3′-UTR, 3′ untranslated region; WT, wild type; Mut, mutant; SD, standard deviation.