Figure 9.

Suppression of IR-induced senescence by recombinant human IL-24 treatment in breast cancer cells. (A) Senescence-associated β-gal and (B) flow cytometric assay of IR-induced senescence in MCF7 cells 3 days after exposure to 4 or 6 Gy of IR. ^*P<0.05, ^**P<0.01. (C) Reverse transcription-polymerase chain reaction analysis of adenoviral-Luc- and adenoviral-Oct4-transduced MCF7 cells probed with IL-24 primer following transfection with siIL-24. IR, ionizing radiation; IL-24, interleukin 24; β-gal, β-galactosidase; Oct4, octamer-binding transcription factor 4; C₁₂FDG, 5-dodecanoylaminofluorescein di-D-galactopyranoside; Luc, luciferase; sicon, control small interfering RNA;siIL-24, small interfering RNA against IL-24.