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. 2018 Apr 18;15(6):9117–9125. doi: 10.3892/ol.2018.8522

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Chrysin induces apoptosis in bladder cancer cells but not non-malignant cells. (A) Human bladder cancer T24 and non-malignant SV-HUC-1 cells were treated with various doses (0, 20, 40, 80 µM) of chrysin for 24 h, then cellular apoptosis was measured by flow cytometry. (B) Human bladder cancer cells T24 and SV-HUC-1 cells were treated with various doses (0, 20, 40, 80 µM) of chrysin for 24 h, then cellular lysates were subjected to western blot analysis. The band densities of western blots were quantitatively analyzed. (C) Human bladder cancer cells T24 and SV-HUC-1 cells were treated with various doses (0, 20, 40, 80 µM) of chrysin for 24 h, then cellular lysates were subjected to caspase activity assays. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01, ***P<0.001 vs. control.

Figure 2. — Chrysin induces apoptosis in bladder cancer cells but not non-malignant cells. (A) Human bladder cancer T24 and non-malignant SV-HUC-1 cells were treated with various doses (0, 20, 40, 80 µM) of chrysin for 24 h, then cellular apoptosis was measured by flow cytometry. (B) Human bladder cancer cells T24 and SV-HUC-1 cells were treated with various doses (0, 20, 40, 80 µM) of chrysin for 24 h, then cellular lysates were subjected to western blot analysis. The band densities of western blots were quantitatively analyzed. (C) Human bladder cancer cells T24 and SV-HUC-1 cells were treated with various doses (0, 20, 40, 80 µM) of chrysin for 24 h, then cellular lysates were subjected to caspase activity assays. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01, ***P<0.001 vs. control.