Figure 3.

Chrysin induces apoptosis via the intrinsic pathway in bladder cancer cells. (A) Human bladder cancer cells T24 were treated with or without chrysin (80 µM) in combination with different caspase inhibitors (Z-LEHD-FMK, Z-IETD-FMK, Z-DEVD-FMK) for 24 h, then cellular apoptosis and viability were measured with flow cytometry and MTT assay, respectively. (B) Human bladder cancer cells T24 were treated with various doses (0, 20, 40, 80 µM) of chrysin for 24 h, and then cellular lysates were subjected to western blot. (C) Human bladder cancer cells T24 and SV-HUC-1 cells were treated with various doses (0, 20, 40, 80 µM) of chrysin for 24 h, then cellular cytosolic fractions were subjected to western blot analysis. The band densities of western blots were quantitatively analyzed. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01, ***P<0.001 vs. control.