Figure 4.
Oldhamianoside II transcriptionally induces Wnt antagonists. Expression levels of Wnt pathway antagonists, including (A) AXIN2, (B) PPP2CB, (C) DKK1 and (D) SFRP5, were evaluated by reverse transcription-quantitative polymerase chain reaction in VCaP cells following oldhamianoside II treatment. The mRNA expression levels of antagonists of the Wnt signaling pathway were normalized to that of GADPH. *P<0.05 and **P<0.01 vs. vehicle control. AXIN2, axin 2; PPP2CB, protein phosphatase 2 catalytic subunit β; DKK1, Dickkopf-1; SFRP5, secreted frizzled-related protein 5.