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. 2018 Apr 23;7(5):727–738. doi: 10.1530/EC-18-0126

Figure 3.

Figure 3

Real-time TaqMan PCR in BeWo and JEG-3 cells after stimulation with T0AM and T1AM. (A) Bar chart of DDC mRNA expression in BeWo cells after incubation with T0AM detected via Real-time TaqMan-PCR. 0.1 nM T0AM induced a significant downregulation of DDC mRNA (P = 0.020). Downregulation of DDC mRNA after incubation with 0.01 nM T0AM was not significant (P = 0.394). b) Bar chart of DDC mRNA expression in BeWo cells after incubation with T1AM detected via Real-time TaqMan PCR. Both 0.001 nM and 0.1 nM T1AM induced a significant downregulation of DDC mRNA expression. (C) Bar chart of DDC mRNA expression in JEG-3 cells after incubation with T1AM detected via Real-time TaqMan PCR. DDC mRNA expression was significantly reduced (P = 0.005) in JEG-3 cells after incubation with 0.01 nM T1AM. For 0.1 nM T1AM, the difference was not significant. DDC, l-dopa decarboxylase.