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. 2018 Apr 13;15(6):5058–5064. doi: 10.3892/etm.2018.6068

Figure 4.

Figure 4.

miR-183 binds directly to MTA1. (A) Schematic of predicted miR-183 binding sites in the MTA1 3′-UTR and the mutant sequences of MTA1. The red text indicates the mutant sequence. (B) MTA1 expression in the miR-183 mimic-transfected, NC and control groups was assessed using western blotting with β-actin as a loading control. (C) A dual luciferase activity was performed to compare osteosarcoma cells transfected with miR-183 mimic and pmirGLO-MTA1 with cells cotransfected with miR-183 negative control and pmirGLO-MTA1. *P<0.05 vs. the miR-183 pGLO-MTA1-mut group. miR, microRNA; MTA1, metastatic tumor protein 1; 3′-UTR, 3′-untranslated region; NC, negative control; wt, wild type; mut, mutant; hsa, human.