Figure 3.

Biological functions of miR-324-5p and −3p arms, as examined in A549 cells. (A) Images of a representative colony formation experiment (4×103) are presented. (B) Following miR-324-5p or −3p mimic transfection, cell proliferation was measured using the CellTiter-Glo® One Solution assay at 0, 1, 2, 3, and 4 days compared with the N.C. (C) The invasion of the cells was examined using a Transwell assay. (D) The invading cells were counted in three fields under a phase-contrast microscope. **P<0.01 and ***P<0.001, with comparisons indicated by lines. miR, microRNA; N.C, negative scramble control.