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. 2018 Apr 16;15(6):9480–9486. doi: 10.3892/ol.2018.8506

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Figure 4. — EHMT1 enhances peritoneal spreading of GC cells in vivo and regulates expression of E-cadherin in GC cell lines and subcutaneous tumors. (A) Representative images of peritoneal spreading and metastasis of BGC-803/NC and BGC-803/sh1 cell xenografts. (B) Quantitative analysis of metastases in each group. (C) Western blotting of E-cadherin in the BGC-803/sh1 and BGC-803/NC groups, with GAPDH used as a loading control. (D) The expression of E-cadherin in the subcutaneous tumor grafts analyzed by immunohistochemistry (magnification, ×200). EHMT1, euchromatic histone lysine methyltransferase 1; GC, gastric cancer; NC, negative control; sh1, EHMT1 short hairpin RNA 1; sh2, EHMT1 short hairpin RNA 2.