Figure 1.

Construction of hepcidin-silenced prostate cancer cells and detection of hepcidin expression. (A) Western blotting results for untransfected and transfected prostate cancer cells. Transfected prostate cancer cells had weak protein bands but untransfected ones had strong bands. (B) Hepcidin protein expression detected by western blotting. Hepcidin expression in prostate cancer cells was significantly higher than that in normal prostate cells, but different prostate cancer cells had similar expression levels. **P<0.01 compared with the normal prostate cells. si, cell lines transfected with small interfering RNAs targeting hepcidin.