Table I.
Gene | Primer sequences (5′-3′) |
---|---|
lncRNA | F: 5′-GGCTCTTCCCTAATGTGTGG-3′ |
HOXD-AS1 | R: 5′-CAGGTCCAGCATGAAACAGA-3′ |
N-cadherin | F: 5′-GGTGGAGGAGAAGAAGACCAG-3′ |
R: 5′-GGCATCAGGCTCCACAGTG-3′ | |
Vimentin | F: 5′-GAGAACTTTGCCGTTGAAGC-3′ |
R: 5′-GCTTCCTGTAGGTGGCAATC-3′ | |
Snail | F: 5′-CCTCCCTGTCAGATGAGGAC-3′ |
R: 5′-CCAGGCTGAGGTATTCCTTG-3′ | |
Slug | F: 5′-GGGGAGAAGCCTTTTTCTTG-3′ |
R: 5′-TCCTCATGTTTGTGCAGGAG-3′ | |
E-cadherin | F: 5′-TGCCCAGAAAATGAAAAAGG-3′ |
R: 5′-GTGTATGTGGCAATGCGTTC-3′ | |
α-catenin | F: 5′-AGCGAATTGTGGCAGAGTGT-3′ |
R: 5′-GTCTACGCAAGTCCCTGGTC-3′ | |
β-catenin | F: 5′-ACAACTGTTTTGAAAATCCA-3′ |
R: 5′-CGAGTCATTGCATACTGTCC-3′ | |
CD44 | F: 5′-TTGCAGTCAACAGTCGAAGAAG-3′ |
R: 5′-CCTTGTTCACCAAATGCACCA-3′ | |
Oct4 | F: 5′-CTTGCTGCAGAAGTGGGTGGAGGAA-3′ |
R: 5′-CTGCAGTGTGGGTTTCGGGCA-3′ | |
CD133 | F: 5′-TGGATGCAGAACTTGACAACGT-3′ |
R: 5′-ATACCTGCTACGACAGTCGTGGT-3′ | |
CD24 | F: 5′-TGAAGAACATGTGAGAGGTTTGAC-3′ |
R: 5′-GAAAACTGAATCTCCATTCCACAA-3′ | |
CD166 | F: 5′-TCCTGCCGTCTGCTCTTCT-3′ |
R: 5′-TTCTGAGGTACGTCAAGTCGG-3′ | |
Lgr5 | F: 5′-TATCTGGCTCCGAGTGCTTGCC-3′ |
R: 5′-ATCATAGCCAGAGATGGATACC-3′ | |
Sox2 | F: 5′-GCCGATGTGAAACTTTTGTCG-3′ |
R: 5′-GGCAGCGTGACTTATCCTTCT-3 | |
Nanog | F: 5′-AATACCTCAGCCTCCAGCAGATG-3′ |
R: 5′-TGCGTCACACCATTGCTATTCTTC-3′ | |
GAPDH | F: 5′-TGCACCACCAACTGCTTAGC-3′ |
R: 5′-GGCATGGACTGTGGTCATGAG-3′ | |
U6 | F: 5′-CTCGCTTCGGCA GCACA-3′ |
R: 5′-AACGCTTCACGAATT TGCGT-3′ | |
miR-217 | F: 5′-TACTCAACTCACTACTGCATCAGGA-3′ |
R: 5′-TATGGTTGTTCTGCTCTCTGTGTC-3′ |
CD, cluster of differentiation; HOXD-AS1, HOXD cluster antisense RNA 1; Lgr5, leucine-rich repeat-containing G-protein coupled receptor 5; lncRNA, long non-coding RNA; miR-217, microRNA-217; Oct4, octamer-binding transcription factor 4; Sox2, sex determining region Y-box 2. F, forward; R, reverse.