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. 2018 May 9;53(1):21–32. doi: 10.3892/ijo.2018.4400

Table I.

Primers used for reverse transcription-quantitative polymerase chain reaction in the present study.

Gene Primer sequences (5′-3′)
lncRNA F: 5′-GGCTCTTCCCTAATGTGTGG-3′
HOXD-AS1 R: 5′-CAGGTCCAGCATGAAACAGA-3′
N-cadherin F: 5′-GGTGGAGGAGAAGAAGACCAG-3′
R: 5′-GGCATCAGGCTCCACAGTG-3′
Vimentin F: 5′-GAGAACTTTGCCGTTGAAGC-3′
R: 5′-GCTTCCTGTAGGTGGCAATC-3′
Snail F: 5′-CCTCCCTGTCAGATGAGGAC-3′
R: 5′-CCAGGCTGAGGTATTCCTTG-3′
Slug F: 5′-GGGGAGAAGCCTTTTTCTTG-3′
R: 5′-TCCTCATGTTTGTGCAGGAG-3′
E-cadherin F: 5′-TGCCCAGAAAATGAAAAAGG-3′
R: 5′-GTGTATGTGGCAATGCGTTC-3′
α-catenin F: 5′-AGCGAATTGTGGCAGAGTGT-3′
R: 5′-GTCTACGCAAGTCCCTGGTC-3′
β-catenin F: 5′-ACAACTGTTTTGAAAATCCA-3′
R: 5′-CGAGTCATTGCATACTGTCC-3′
CD44 F: 5′-TTGCAGTCAACAGTCGAAGAAG-3′
R: 5′-CCTTGTTCACCAAATGCACCA-3′
Oct4 F: 5′-CTTGCTGCAGAAGTGGGTGGAGGAA-3′
R: 5′-CTGCAGTGTGGGTTTCGGGCA-3′
CD133 F: 5′-TGGATGCAGAACTTGACAACGT-3′
R: 5′-ATACCTGCTACGACAGTCGTGGT-3′
CD24 F: 5′-TGAAGAACATGTGAGAGGTTTGAC-3′
R: 5′-GAAAACTGAATCTCCATTCCACAA-3′
CD166 F: 5′-TCCTGCCGTCTGCTCTTCT-3′
R: 5′-TTCTGAGGTACGTCAAGTCGG-3′
Lgr5 F: 5′-TATCTGGCTCCGAGTGCTTGCC-3′
R: 5′-ATCATAGCCAGAGATGGATACC-3′
Sox2 F: 5′-GCCGATGTGAAACTTTTGTCG-3′
R: 5′-GGCAGCGTGACTTATCCTTCT-3
Nanog F: 5′-AATACCTCAGCCTCCAGCAGATG-3′
R: 5′-TGCGTCACACCATTGCTATTCTTC-3′
GAPDH F: 5′-TGCACCACCAACTGCTTAGC-3′
R: 5′-GGCATGGACTGTGGTCATGAG-3′
U6 F: 5′-CTCGCTTCGGCA GCACA-3′
R: 5′-AACGCTTCACGAATT TGCGT-3′
miR-217 F: 5′-TACTCAACTCACTACTGCATCAGGA-3′
R: 5′-TATGGTTGTTCTGCTCTCTGTGTC-3′

CD, cluster of differentiation; HOXD-AS1, HOXD cluster antisense RNA 1; Lgr5, leucine-rich repeat-containing G-protein coupled receptor 5; lncRNA, long non-coding RNA; miR-217, microRNA-217; Oct4, octamer-binding transcription factor 4; Sox2, sex determining region Y-box 2. F, forward; R, reverse.