Effect of CIEE on protein expressions of PPARα, C/EBPα and FAS in liver tissue of HFD fed mice. Protein levels of regulator transcription factors of adipogenesis and FAS in liver tissues were assessed by western blot. (A) Immunoblot analysis and bar diagram of the relative band intensities of (B) PPARα (C) C/EBPα (D) FAS in liver tissue of HFD-fed obese mice. Results are means ± SEM (n=6). aP<0.05 vs. ND group, bP<0.05 vs. HFD group. ND, normal diet group; HFD, high-fat diet group; CIEE, ethanol extract of Chrysanthemum indicum L.; GC, Garcinia cambogia; PPAR, proliferator-activated receptor; C/EBPα, CCAAT/enhancer-binding protein-α; FAS, fatty acid synthesis.