Figure 1.

Efficacy of OOS on SCLC cells in vitro. (A) Dose-dependent effect of OOS on the proliferation of GLC-8 cells was assessed in vitro. Cells were incubated with OOS at the indicated dilution factors and MTT metabolization was measured as indicated. (B) Calculation of the IC₅₀ of OOS. Mean absorbance values of the untreated samples were considered as 100% and the mean values were referred to the control value. (C and D) Similarly, dose-dependent effect of OOS on DMS 92 cells was determined (C) and the IC₅₀ value was calculated (D). (E and F) The effect of OOS alone (1:100) or in combination with vincristine [VCR 5 nM (E)] or docetaxel [TXT 5 nM (F)] were determined by MTT assays. The mean absorbance values of the untreated samples were considered as 100%. Data are represented as mean ± SD of quadruplicates of an experiment that was repeated at least twice. OOS, Ocoxin^® oral solution; SCLC, small cell lung cancer; IC₅₀, half maximal inhibitory concentration.