Table II.

Sequence quality control results for 3 hereditary non-polyposis colorectal cancer patients.

Sample name	Orientation	Total reads, n	Total bases, n	Q20, %	Depth	1×, %	Quality of sequencing results
Lah-1	Forward	48,356,713	4,835,671,300	98	105.69	99.95	Good
Lah-2	Reverse	48,356,713	4,835,671,300	97			Good
Lyl-1	Forward	73,411,952	7,341,195,200	97	149.87	99.86	Good
Lyl-2	Reverse	73,411,952	7,341,195,200	95			Good
Lyh-1	Forward	57,778,031	5,777,803,100	97	120.25	99.96	Good
Lyh-2	Reverse	57,778,031	5,777,803,100	95			Good

The Q20 value refers to the probability of error given to the identified base in the base calling process. If the mass value is Q20, the probability of error recognition is 1%, that is, the error rate is 1% or the correct rate is 99%. The 1× value refers to the likelihood that there is at least one read coverage in the genome sequence.