Comparison of the relative APOE methylation between ASD cases and healthy controls. (A) Comparison between ASD cases and controls. The median PMR in cases and controls was 33% (interquartile range, 25 and 37%) and 11% (interquartile range, 6 and 23%), respectively. The P-value for the comparison between the cases and the controls was 2.36×10−10. (B) Comparison between male ASD cases and male controls. The median PMR values of cases and controls were 32% (23, 37%) and 11% (6, 27%), respectively. The P-value for the comparison between the male cases and the male controls was 8.52×10−10. (C) Comparison between female ASD cases and female controls. The median PMR values of cases and controls were 0.42 (0.29, 0.56) and 0.08 (0.05, 0.10), respectively. The P-value for the comparison between the female cases and the female controls was 4.18×10−6. APOE, apolipoprotein E; ASD, autism spectrum disorder; PMR, percentage of methylation of a reference.