Figure 7.

KRAS-mutant lung adenocarcinoma cells are slightly dependent on TTF-1 for proliferation. (A) Effects of TTF-1 knockdown on the proliferation of H358 and H441 cells. Cells were transfected with NC siRNA or NKX2-1 siRNA (10 nM each) and cultured for 48 h. Viable cells were estimated for another 96 h in triplicate. Results are shown as the means ± SD; ^**P<0.01. (B) Western blot analysis of the effects of TTF-1 knockdown in H358 and H441 cells. Cells were transfected with NC siRNA or NKX2-1 siRNA (10 nM each) and then cultured for 72 h. (C) Quantification of survivin expression, as shown in (B), in NC siRNA- or NKX2-1 siRNA (10 nM each)-transfected H358 and H441 cells. Columns, mean (n=3); bars, ± SD; ^*P<0.05, ^**P<0.01.