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. 2018 May 15;6:e4774. doi: 10.7717/peerj.4774

Figure 1. Melanocortin-1 receptor (MC1R) expression on rat brain endothelial cells and isolated rat brain microvessels.

Figure 1

(A) Cells were grown in culture dish and brain microvessels were isolated from adult rat brain. Total RNA was extracted, and reverse transcription and PCR amplification were performed for Mc1r. Actin gene was used as reference. The predicted length of the PCR products was 160 bps. Fragments were analyzed on MetaPhor agarose gel (3%) next low range DNA marker. (B) Cultured brain endothelial cells were grown on culture inserts and immunostained for MC1R. Cell nuclei were labeled with ethidium homodimer-1 (red). Green: MC1R labeling. Scale bar: 10 μm. (C) Isolated brain microvessels were immunostained for MC1R. Fluorescent labeling is shown together with differential interference contrast to reveal the structure of brain capillary. Cell nuclei were labeled with ethidium homodimer-1 (red). Green: MC1R labeling. Scale bar: 5 μm. MC1R, melanocortin-1 receptor; RBEC, rat brain endothelial cells; MV, microvessels.