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. 2018 Apr 10;15(6):8991–8998. doi: 10.3892/ol.2018.8455

Figure 1.

Figure 1.

Proliferation of BCap37 cells is not affected by the expression of Bcl-2 in vivo or in vitro. Results of reverse transcription-quantitative polymerase chain reaction (left) and western blot (right) analyses showed that (A) Bcl-2 was expressed at high levels in BC-Bcl2 cells, compared with its levels in BC-Mock cells, and (B) Bcl-2 was effectively silenced in the BC-shRNA-Bcl2 cells, compared with the BC-shRNA-NC cells. Protein levels were quantitated by densitometry. (C) CCK8 assay revealed no statistically significant difference in relative cellular viability of BCap37 cells upon Bcl-2 overexpression or silencing, compared with the control. (D) Tumor growth was not affected by Bcl-2 in BCap37 tumor xenografts. Nude athymic mice were implanted with BCap37, BC-shRNA-NC, BC-shRNA-Bcl2, BC-Mock, and BC-Bcl2, respectively (n=5 for each group). The tumor growth curves in each group are shown. The data are presented as the mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01, ***P<0.001. BC, BCap37 cell; Bcl-2, B-cell lymphoma 2; shRNA, short hairpin RNA; NC, negative control; CCK8, Cell Counting kit-8; n.s., not significant.