Figure 4.
Overexpression of Bcl-2 in BCap37 cells induces the progression of epithelial-to-mesenchymal transition. Western blot assay of cells expressing BC-Mock BC-Bcl2, BC-shRNA-NC or BC-shRNA-Bcl2. Quantification showed that the overexpression of Bcl-2 inhibited the expression of E-cadherin, but increased the levels of N-cadherin and vimentin. The inhibition of Bcl-2 increased the expression of E-cadherin, but inhibited that of N-cadherin and vimentin. Protein levels were quantified by densitometry. BC, BCap37 cell; Bcl-2, B-cell lymphoma 2; shRNA, short hairpin RNA; NC, negative control.