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Thermocycling steps for B-cell lymphoma 2 gene amplification.
| 98°C | 2 min (Ramp rate: 4.4°C/sec) | 1 cycle |
| 98°C | 10 sec (Ramp rate: 4.4°C/sec) | 35 cycles |
| 55°C | 15 sec (Ramp rate: 2.2°C/sec) | |
| 72°C | 15 sec (Ramp rate: 2.2°C/sec) | |
| 72°C | 10 min (Ramp rate: 2.2°C/sec) | 1 cycle |
| 4°C | 1 min (Ramp rate: 2.2°C/sec) | 1 cycle |
