Figure 7.

MORC2 regulates liver cancer cell proliferation via cell cycle and apoptosis. (A) Effects of MORC2-knockdown on the percentage of cells in the S/G2/M-phase were determined by FACS analysis in HepG2 (left), Bel-7402 (middle) and LM3 (right) cells. (B) Following Annexin V-FITC/propidium iodide staining of the indicated cells following treatment without or with H₂O₂ (1 mmol) for 12 h, effects of MORC2-knockdown on HepG2 cell apoptosis were determined by FACS analysis (above) and quantitative analysis of apoptotic cell numbers (below). Data are presented as the mean ± standard deviation of three independent experiments. ^**P<0.01; ^***P<0.001. FACS, fluorescence-activated cell sorting; MORC2, microrchidia 2; si, small interfering RNA; CON, control; KD, knockdown; NC, negative control.