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. 2018 Mar 27;53(1):59–72. doi: 10.3892/ijo.2018.4333

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Copyright: © Pan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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MORC2 modulates migration, invasion and metastasis of liver cancer cells in vitro. (A) Cell migration was assessed using a Transwell assay in HepG2 (left), Bel-7402 (middle) and LM3 (right) cells following transfection of the cells with MORC2 siRNA for 48 h. The cells that migrated into the bottom surface of the filters were stained. Magnification, ×40. (B) Cell invasion was assessed using a Transwell assay with Matrigel in HepG2 (left), Bel-7402 (middle) and LM3 (right) cells following transfection of the cells with MORC2 siRNA for 48 h. The cells that invaded into the lower surface of the filters were stained. Magnification, ×40. (C) Movement ability was detected by scratch wound healing assays in HepG2 (left), Bel-7402 (middle) and LM3 (right) cells following transfection of the cells with MORC2 siRNA for 48 h. Magnification, ×40. Data are presented as the mean ± standard deviation of six independent experiments. ^***P<0.001. MORC2, microrchidia 2; siRNA, small interfering RNA; CON, control; KD, knockdown; NC, negative control.