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. 2018 Mar 27;53(1):59–72. doi: 10.3892/ijo.2018.4333

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Copyright: © Pan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Overexpression of MORC2 promotes proliferation of SMMC7721 cells in vitro. (A) Overexpression of MORC2 in SMMC7721 cells by lentivirus-mediated packed pLV-MORC2 vector was confirmed by western blot analysis; β-actin was used as a loading control. (B) Effect of overexpression of MORC2 on the proliferation on SMMC7721 cells was determined using a cell counting kit-8 assay. (C) Effect of overexpression of MORC2 on colony numbers was determined by a colony formation assay in SMMC7721 cells. (D) Effect of overexpression of MORC2 on the percentage of cells in the S/G2/M-phase and on (E) SMMC7721 cell apoptosis were determined by fluorescence-activated cell sorting analysis. Data are presented as the mean ± standard deviation of three or six independent experiments. ^*P<0.05; ^**P<0.01; ^***P<0.001. MORC2, microrchidia 2; CON, control.