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. Author manuscript; available in PMC: 2018 May 18.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Sep 6;36(3):517–522. doi: 10.1111/j.1530-0277.2011.01606.x

Figure 2. Anticatalase-Lentiviral vector injection into the ventral tegmental area (VTA) inhibited acetaldehyde generation by VTA homogenates.

Figure 2

VTA homogenates from UChB rats pretreated with a single dose (8×104 virus) of anticatalase-Lenti-shRNA vector injected locally into the VTA, 30 days before the experiment, produced a significantly (p<0.001) lower concentration (−75%) of acetaldehyde via ethanol oxidation than VTA homogenates from rats pretreated with empty lentiviral vector (control-Lenti). Incubations were carried out for 60 min at 37° C in the presence of 50 mM ethanol 10 mM glucose and VTA homogenate (5 mg wet tissue). At 10 mM, 3-amino-1,2,4-triazole (AT), a catalase inhibitor, blocked by 80% the formation of acetaldehyde by the homogenates. Acetaldehyde generated was therefore adjusted to reflect only that due to catalase activity. See Experiment 2 in Methods. Data shown are means ± S.E.M.