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. Author manuscript; available in PMC: 2018 Jul 19.
Published in final edited form as: Science. 2018 Jan 19;359(6373):eaah6834. doi: 10.1126/science.aah6834

Fig. 3. Release of full-length β-barrel precursor from the SAM complex.

Fig. 3

(A) [35S]Por1C276 constructs of different length were incubated with isolated Sam50Cfree or Sam50C130 mitochondria for 30 min, followed by oxidation with 4-DPS, analysis by non-reducing SDS-PAGE and autoradiography. Lanes 27 and 28, shorter exposure to compensate for strong intensity of full-length Por1. Arrowheads, disulfide-bonded Sam50-precursor adducts; circles, Por1C276 precursor. (B and C) Samples as described for (A) were analyzed by blue native electrophoresis and autoradiography. Lanes 1–4 vs. lanes 5–12 of (B), linear adjustment of brightness and contrast to compensate for strong intensity of Por1(β15-19)C276. POR, assembled Porin complex; SAM-Por1, Porin1 precursor at SAM.