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. Author manuscript; available in PMC: 2018 Jul 19.
Published in final edited form as: Science. 2018 Jan 19;359(6373):eaah6834. doi: 10.1126/science.aah6834

Fig. 7. β-Barrel precursors in transit are in close proximity to Sam50 loop 6.

Fig. 7

(A) [35S]Por1(β14-19) precursors carrying a cysteine in β-strand 14 at position 205 or 206 and [35S]Por1(β12-19) precursors carrying a cysteine in β-strand 12 at position 180 or 179 were imported for 5 min into isolated mitochondria containing Sam50 variants, followed by crosslinking with BMH. Samples were analyzed by non-reducing SDS-PAGE and autoradiography. Arrowheads, cysteine-specific Sam50-Por1 precursor crosslinking products. (B) Schematic model summarizing the crosslinking results of Fig 7C and fig. S9, B and C. (C) Radiolabeled Por1 constructs were imported into mitochondria of yeast strains expressing the indicated Sam50 variants for 5 min. Samples were treated with BMH and analyzed as described in (A).