Fig. 6.

DARPP-32 knockdown decreases tumor progression in a human lung tumor xenograft model. a Luciferase-labeled human A549, b H1650 and c H226 cells transduced with lentivirus encoding control or DARPP-32 shRNAs were injected into the left thorax of SCID mice and imaged for luminescence on indicative days. d Luciferase-labeled human A549 cells overexpressing control, DARPP-32 or t-DARPP clones were orthotopically injected into the left thorax of SCID mice and imaged for luminescence on indicative days. Representative in vivo images of SCID mice are depicted. Total luminescence intensity (photon count) was calculated using molecular imaging software. The colored bar represents the numerical value of luminescence. Error bars indicate SEM. *P < 0.05, ***P < 0.001 and ****P < 0.0001, one-way ANOVA followed by Dunnett’s test for multiple comparison