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. Author manuscript; available in PMC: 2019 May 15.
Published in final edited form as: Immunity. 2018 May 15;48(5):963–978.e4. doi: 10.1016/j.immuni.2018.04.017

Figure 2. Gαq, phospholipase Cβ, and protein kinase Cη function downstream of muscarinic receptors.

Figure 2

(A) Epifluorescence micrographs of clec-60p::gfp animals reared on E. coli carrying empty vector or RNAi against egl-8, pkc-1, or egl-30, and subsequently infected with S. aureus for 16 h. (B) Quantitative analysis of (A). Data are mean ± SEM (at least 2 independent biological replicates, n ≥ 50 per condition). (C) qRT-PCR of clec-60 in wild type animals and egl-8 mutants after 8 h S. aureus infection. Results are normalized to non-infected wild type. Data are mean ± SEM (three independent biological replicates, n ≥ 3,000 per condition). (D) Representative epifluorescence micrographs of clec-60p::gfp animals reared on E. coli carrying empty vector or RNAi against egl-30 or pkc-1, and subsequently treated with 5 mM arecoline for 16 h. (E) Quantitative analysis of (D). Data are mean ± SEM (at least two independent biological replicates, n ≥ 50 per condition) (F) qRT-PCR of clec-60 in wild type and egl-8 mutant animals after 8 h treatment with 1 mM arecoline. Results are normalized to vehicle treated wild type. Data are mean ± SEM (three independent biological replicates, n ≥ 3,000 per condition). (G) Survival of wild type and egl-8 mutant animals infected with S. aureus. Results are representative of 2 independent biological replicates. (H) Lifespan of wild type and egl-8 mutant animals on E. coli OP50. Results are representative of 2 independent biological replicates. (I) Survival of wild type and pkc-1 mutant animals infected with S. aureus. Results are representative of 2 independent biological replicates. (J) Lifespan of wild type and pkc-1 mutant animals on E. coli OP50. Results are representative of 2 independent biological replicates. ns, p > 0.05. (K) Schematic summary of results. Scale bars, 0.6 mm.