Figure 7. Transcription factors that link muscarinic and Wnt signaling.

(A) Quantification of GFP expression in clec-60p::gfp animals subjected to RNAi against the indicated transcription factor genes, and subsequently treated with 5 mM arecoline for 16 h. Data are mean ± SEM (2 independent biological replicates, n ≥ 50 per condition). Results are normalized to vehicle-treated empty vector control. (B) Quantification of Venus expression in cwn-2p::cwn-2::venus animals treated with RNAi as in (A) and subsequently with 5 mM arecoline for 24 h. Data are mean ± SEM (2 independent biological replicates, n ≥ 50 per condition). Results are normalized to vehicle-treated empty vector control. (C) qRT-PCR of clec-60 in wild type and lin-1 null mutant animals treated with 5 mM arecoline for 8 h. Results are normalized to vehicle-treated wild type control. Data are mean ± SEM (3 independent biological replicates, n ≥ 3,000 per condition). (D) qRT-PCR of cwn-2 in the same animals as in (C), treated with 5 mM arecoline for 16 h. (E) qRT-PCR of clec-60 in E. coli-fed wild type and lin-1 gain of function (GF) mutant animals. Results are normalized to wild type. Data are mean ± SEM (3 independent biological replicates, n ≥ 3,000 per condition). (F) qRT-PCR of cwn-2 in the same animals as in (E). (G) MGH167 animals subjected to RNAi against lin-1 until young adulthood, and subsequently infected with S. aureus. Results are representative of 2 independent biological replicates. (H) Lifespan of MGH167 animals treated as in (G), but placed on E. coli OP50. Also see Figure S5.