Fig 5. Titan cells generation in vitro is influenced in vitro by host derived cues and ergosterol, protein and RNA inhibitors.

(A) Monoclonal anti-cryptococcal capsular polysaccharide antibodies E1 and 18B7 (at 166 μg/mL in MM) significantly decreased cell size, as fetal calf serum (FCS, 5%) (B) and phosphatidylcholine at 5mM (C) did. (D) Fluconazole; (E) flucytosine at concentration below the MIC (4 mg/L) and (F) cycloheximide at 0.1μg/mL drastically impaired titan cells formation with almost no titan cells produced upon drug exposure.