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. 2018 May 18;14(5):e1006982. doi: 10.1371/journal.ppat.1006982

Fig 7. Titan cells generation is dependent on various genes and requires signaling through the Gpr/PKA/Rim101 pathway in vitro.

Fig 7

(A) The different H99 strains harbored variable abilities to produce titan cells compared to H99O (grey bar) with high titan cells producer (H99O, S, L) and low titan cells producer (KN99α, H99W, H99 CMO18) in vitro. (B) Sgf29Δ and lmp1Δ deletion mutants show a decrease in titan cells generation in various H99 backgrounds in vitro compared to H99O. Complementation in strains lmp1Δ:LMP1 and sgf29Δ:SGF29 in H99S background restored the phenotype of H99S. Rim101 (C) and GPR4 and GPR5 and CAC1 (D) are required for titan cells generation in vitro in H99 and KN99α. The ratio to the value obtained for H99O used as a calibrator in each experiment was calculated for each strain. Bar represent mean ± SD (mean cell counted = 600). Khi2 test was performed to compare the experimental conditions to H99O, they were performed in triplicates and pooled (*p<0.0001, **p<0.0001, when the comparison was done with the parental strain H99S).