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. 2018 Apr 1;32(7-8):497–511. doi: 10.1101/gad.311027.117

Figure 2.

Figure 2.

Glucose-dependent cell migration requires ACLY. (A) Wound healing assay in U251 with stable knockdown of ACLY. (*) P < 0.05. (B) Relative adhesion in U251 cells with knockdown of ACLY. (**) P < 0.01; (****) P < 0.0001. (C) Transwell migration of LN229 cells after treatment with 50 µM ACLY inhibitor (ACLYi; BMS303141) or vehicle in 10 mM glucose. No FBS is a negative control. (**) P < 0.01. (D) Adhesion assay in with U251 cells after treatment with 50 µM ACLYi or vehicle for 24 h. (E) Relative mRNA expression of acetyl-CoA-regulated genes in LN229 cells treated with 50 µM ACLYi. (###) P < 0.001; (####) P < 0.0001; (**) P < 0.01; (***) P < 0.001; (****) P < 0.0001. (F) Relative adhesion in two different ACLY knockout clones (sg3.6 and sg3.8) on 1% fibronectin. Parental cells were infected with vector expressing Cas9 but without guide RNA. (**) P < 0.01; (****) P < 0.0001. (G) Relative expression of ITGA2B in cells described in F. (*) P < 0.05; (**) P < 0.01. All panels show mean ± SEM.