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. 2018 Apr 1;32(7-8):497–511. doi: 10.1101/gad.311027.117

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© 2018 Lee et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — NFAT1 nuclear localization is acetyl-CoA-regulated and required for cell adhesion. (A) Immunofluorescent detection of endogenous NFAT1 localization in LN229 cells. Representative images are shown. (B) Quantification of NFAT1 detected in the nucleus, as shown in A, as percentage of cells in each field. At least 170 cells were scored per condition. (**) P < 0.01. (C) Immunofluorescent detection of endogenous NFAT1 localization in ACLY knockout sg3.6 cells with or without murine wild-type ACLY cDNA expression. Representative images are shown. (D) Quantification of endogenous NFAT1 detected in the nucleus, as shown in C, as percentage of cells in each field. (**) P < 0.01; (***) P < 0.001; (****) P < 0.0001. (E) Murine HA-tagged NFAT1 was transiently expressed in LN229 cells, cells were treated with the indicated doses of ACLYi, and nuclear and cytosolic fractions were prepared and analyzed by Western blot. (F) Glucose regulation of cell adhesion on 1% fibronectin in LN229 cells expressing empty vector, murine wild-type NFAT1, or constitutively active (CA) NFAT1 (CA-NFAT1). (G) ACLYi regulation of cell adhesion on 1% fibronectin in cells expressing empty vector (plx303), wild-type NFAT1, or CA-NFAT1. (*) P < 0.05; (**) P < 0.01; (****) P < 0.0001.