Figure 6.

Ca²⁺ signaling is acetyl-CoA-regulated. (A) Representative Ca²⁺ traces from the GCaMP6f reporter in the indicated conditions. Each trace represents one cell, and at least 15 cells were analyzed from two different fields in each condition. (B) Quantification of Ca²⁺ spikes after nutrient add-back. At least 116 cells were scored in each condition in a blinded manner. (*) P < 0.05; (**) P < 0.01. (C) Representative Ca²⁺ traces in the presence or absence of EGTA are shown. (D) LN229 cell adhesion onto 1% fibronectin after 20 h of incubation in the indicated concentrations of glucose and acetate followed by 4 h of treatment with 0.5 µM ionomycin or vehicle control. (****) P < 0.0001. (E) Relative adhesion after 20 h of treatment with ACLYi followed by 4 h of treatment with 0.5 µM ionomycin or vehicle control in LN229 cells expressing murine wild-type NFAT1. (*) P < 0.05; (**) P < 0.01. (F) Model showing how acetyl-CoA promotes GBM cell adhesion and migration through Ca²⁺–NFAT signaling.