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. 2018 Jun;24(6):761–768. doi: 10.1261/rna.065508.117

FIGURE 3.

FIGURE 3.

Blocking CA repeats mimics depletion of hnRNP L in inclusion of the SH3BGRL cryptic exon. (A) Sequence of the SH3BGRL cryptic exon (uppercase) and flanking intron (lowercase) with sequence complementary to CA blocking AMO (underlined). CA dinucleotides are in bold. (B) RT-PCR gel showing inclusion of SH3BGRL cryptic exon upon transfection of CA blocking AMO, hnRNP L-depleting AMO or an unrelated GT-rich AMO. Western blot confirming depletion of hnRNP L upon treatment with the hnRNP L AMO, but not others, shown on right. HnRNP L depletion by AMO was performed as described previously (Cole et al. 2015). Translation blocking AMO for hnRNP L was used at 2, 5, and 10 nmol. CA and nonspecific AMO were used at 5, 10, and 20 nmol.