FIGURE 11.
Maternal transmission of antimiR-29a-3p to mouse pups increases steady-state expression levels of miR-29a-3p target-genes. Either a scrambled antimiR (open symbols) or an antimiR directed against miR-29a-3p (closed symbols) was administered by intravenous (i.v.) injection at a dose of 100 mg kg−1 to pregnant dams on the 17th day of gestation. The liver was harvested from mouse pups on postnatal day (P)7. Steady-state mRNA expression levels were assessed by real-time RT-PCR for the miR-29a-3p targets (A) Eln, and (C) Adamts7, described by the mean ΔCT ± SD. (n = 4–6 animals per group; each symbol represents an animal, where some symbols may coalesce), where ΔCT = CT(Polr2a) − CT(gene of interest). Mean values were compared between treatment conditions by unpaired Student's t-test. P-values are provided when P < 0.05. Total liver protein extracts from pups at P7 were screened by immunoblot for steady-state protein expression levels of (B) elastin (ELN) and (D) ADAM metallopeptidase with thrombospondin type 1 motif 7 (ADAMTS7), where β-ACTIN served as a loading control. For immunoblots, protein extracts from three scrambled antimiR-treated mice and three antimiR-29a-3p-treated mice were run side-by-side, on the same gel.