FIGURE 6.
Steady-state levels of miR-29a-3p in mouse pups are influenced by administration of antimiR-29a-3p to pregnant dams. Either a scrambled antimiR (open symbols) or an antimiR directed against miR-29a-3p (closed symbols) was administered by intraperitoneal (25 mg kg−1) or intravenous (i.v.; 25 or 100 mg kg−1) injection to pregnant dams on the 17th day of gestation. Organs were harvested from mouse pups, either on the day of birth, postnatal day (P)1, or at P7 or P14: (B) brain, (H) heart, (K) kidney, (Li) liver, (Lu) lung. Total RNA pools were screened by real-time RT-PCR for steady-state miR-29a-3p levels in organs from pups at (A) P1, (B) P7, and (C) P14. The miR steady-state expression levels are described by the mean ΔCT ± SD. (n = 4–6 animals, per group; each symbol represents an individual animal), where ΔCT = CT(Rnu6) − CT(miR-29a-3p). Mean values were compared between the scrambled versus miR-29a-specific antimiR-treated mouse pups by unpaired Student's t-test. (*) P < 0.05; (**) P < 0.01; (***) P < 0.001; (****) P < 0.0001.