FIGURE 7.

AntimiR-29a-3p was detected in selected organs of pups born to antimiR-29a-3p-treated dams by stem–loop real-time RT-PCR. Scrambled antimiR or antimiR-29a-3p was administered (100 mg kg⁻¹, via the intravenous route) to pregnant dams on gestational day 17. The antimiR-29a-3p was detected by stem–loop real-time RT-PCR in total RNA pools from the lungs, liver, and kidneys of offspring at postnatal day 7. The data reflect the mean fold-change in the abundance of PCR product detected by stem–loop real-time RT-PCR, relative to miR-16, and normalized to the scrambled antimiR-treated group, as described in Materials and Methods.