Figure 1.

Optimizing reconstitution buffer for dried tIPV followed by analysis in D-antigen potency assay. Trivalent IPV was dried on a liquid crystal polymer (LCP) discs, and various buffers containing different concentrations of PS80 and BSA were tested to reconstitute the dried tIPV samples followed by analysis in the D-antigen potency assay either (a) immediately after drying, or (b) after storage in dried state for 7 days at 4°C. (c) the key describing each of the solution conditions examined as reconstitution buffers. Each condition is shown as the relative D-antigen potency of each IPV serotype as compared with a liquid tIPV stock solution (100% potency), and error bars represent the SD (1 SD) from quadruplicate experiments.