Two-week tIPV stability study with 2 candidate formulations in an M199/DPBS base buffer. Trivalent IPV was dried and stored in 2 candidate formulations (F1 [black]: 4.5% SBE-β-cyclodextrin + 15-mM glutathione, and F2 (blue): 2.5% γ-cyclodextrin + 15-mM glutathione) or DPBS alone (red) at either 4°C (solid lines) or 25°C (dashed lines) for 6 h, and 1, 3, 7, or 14 days. Relative D-antigen potency loss was measured in each IPV serotype (a) IPV1, (b) IPV2, (c) IPV3 and is relative to a liquid tIPV stock solution (0% potency loss). T0 represents each IPV serotypes potency loss immediately after drying. Excipient samples were prepared in a M199/DPBS base buffer (see Materials and Methods section). Error bars represent the SD (1 SD) from triplicate experiments.