The combination temsirolimus and RG7388 strongly inhibits neuroblastoma tumor growth. A, LAN5 MYCN-amplified xenografts were treated with vehicle, single-agent temsirolimus (10 mg/kg), single agent RG7388 (25 mg/kg), and their combination. Average tumor weights for each cohort ± SEM are shown (n = 10 in each group). Tumors treated with dual therapy are significantly smaller than those treated with single therapy. B, SH-SY5Y MYCN nonamplified xenografts received treatment as described above (A; n = 10 in each group). Tumors treated with dual therapy are significantly smaller than those treated with single therapies.