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. 2017 Dec 31;14(4):654–670. doi: 10.1080/15548627.2017.1338556

Table 1.

Primary antibodies used in this study.

Primary Antibody
Dilution Ratio
Supplier
ATG5 (rabbit polyclonal) 1:1,000 Cell Signaling Technology, 2630
ACTB (mouse monoclonal) 1:3,000 Sigma-Aldrich, A-3853
BECN1(rabbit polyclonal) 1:2,000 Cell Signaling Technology, 3738
CASP3 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 9665
CDKN1A/p21Waf1 (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 9932
CDKN1B/p27Kip1(rabbit monoclonal) 1:2,000 Cell Signaling Technology, 9932
CDKN2A/p16INK4a (rabbit monoclonal) 1:2,000 Abcam, ab108349
CTSB (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-365558
CTSD (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-377299
Cleaved CASP3 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 9664
Cleaved CASP7 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 8438
Cleaved CASP9 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 7237
FLAG (mouse monoclonal) 1:4,000 Sigma-Aldrich, F3165
GAPDH (rabbit polyclonal) 1:3,000 Trevigen, 2275
HA (rabbit monoclonal) 1:2.000 Cell Signaling Technology, 3724S
LC3B (rabbit polyclonal) 1:2,000 Sigma-Aldrich, L7543
MTOR (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 2983
NUPR1 (mouse monoclonal) 1:1,000 Abcam, ab87454
p-MTOR(S2448) (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 5536
PCNA (rabbit polyclonal) 1:1,000 Abcam, ab18197
SNAP25 (rabbit polyclonal) 1:1,000 Abcam, ab109105
SQSTM1/p62 (rabbit polyclonal) 1:1,000 Cell Signaling Technology, 7695
VAMP8 (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-166820