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. 2018 Feb 21;14(4):567–583. doi: 10.1080/15548627.2018.1429874

Figure 4.

Figure 4.

Pharmacological treatments rescue autophagic activity and ciliary length in the absence of RPGRIP1L. (A to C) Immunofluorescence-based cilia length quantifications. (A) Measurement of ciliary length after rapamycin treatment. The ciliary length in wild-type and Rpgrip1l-deficient MEFs is decreased after application of rapamycin (n = 3). (B) Application of the autophagy inhibitor 3-MA lengthens cilia in wild-type MEFs, but not Rpgrip1l-deficient MEFs. The ciliary axoneme is marked by Ac-TUBA (green), the basal body by TUBG (blue) (n = 4 for wild-type; n = 5 for rpgrip1l−/− MEFs). Scale bar: 1 µm. (C) Application of the autophagy activator ABT-737 decreases ciliary length in wild-type and Rpgrip1l-deficient MEFs. The ciliary axoneme is stained with Ac-TUBA (green), the basal body with TUBG (blue) (n = 4 for wild-type; n = 5 for Rpgrip1l−/− MEFs). Scale bar: 1 µm. Asterisks indicate statistically significant differences. The most important significant differences are written in bold. (A and C) Cells were treated with DMSO as a vehicle control.