Author response image 2. Western blots showing expression levels and lifetimes of Oct4, Sox2, Nanog, Lin28, and MyoD1 proteins in MRC-5 human fetal lung fibroblasts transfected with protein- encoding RNA, relative to levels in hES (H9) and rhabdomyosarcoma (Rh30) cells.

b-actin was used as a loading control. Left panels: The amount of RNA per 50 mL electroporation volume was varied as indicated. Cells were lysed 6 hours after transfection. Right panels: Cells were transfected with 1 mg of RNA, and lysed at the indicated times. (Reproduced from Angel and Yanik, PLoS ONE 2010 under the Creative Commons Attribution License https://doi.org/10.1371/journal.pone.0011756)